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Online qualification course for DOT-Regulated Urine Collection

Qualification training for DOT-regulated urine specimen collections

Department of Transportation (DOT) regulated urine specimen collections are an important part of employment and workplace screenings. As a collector, you'll perform DOT urine specimen collections for drug and alcohol testing, return-to-duty testing, and more.

Get qualification training in DOT urine collection

In order to perform DOT-regulated urine specimen collections, you'll need to complete qualification training. MediaLab's online course in Department of Transportation (DOT) Regulated Urine Specimen Collection satisfies the requirements for qualification training as outlined in Federal Regulations, 49 CFR Part 40.

Key topics in MediaLab's qualification training include monitored and observed collections, custody and control forms, donor identification, procedures for urine collection, and how to address common problems that may occur during collections.

An affordable online solution for qualification training

MediaLab's online course in Department of Transportation (DOT) Regulated Urine Specimen Collection includes an online, self-paced qualification training course and a tracking feature for documenting the five required mock collections (see details below). For just $45, you'll be able to access the qualification training and tracking features for a full year.


What does this course include?

This course includes three components:

  1. Online reading material covering DOT rules and procedures (about 1 - 2 hours to cmplete)
  2. A short exam covering the reading material (about 10 - 15 minutes to complete)
  3. A checklist to record completion of five mock collections (time varies)

How to become qualified to perform DOT urine collections

There are two steps to becoming qualified for DOT urine collection:

  1. Complete a qualification training course, like this online course from MediaLab.
  2. Complete five mock collections under various scenarios in the presence of a qualified observer.

MediaLab's online DOT urine collection training course includes an integrated checklist feature to document completion of these required mock collections, but MediaLab does not provide observers for the mock collections. You will need to locate a qualified collector locally who can attest that you have completed the mock collections.

Who can observe the mock collections for DOT qualification training?

A qualified collector must perform the observations. A qualified collector must either (a) regularly perform DOT-regulated urine specimen collections, (b) regularly observe collections for DOT training requirement, or (c) have completed a "train the trainer" course. Observations must be conducted in person by a qualified collector and cannot be performed online or remotely.

How do I find an observer?

This course is normally used by hospitals, laboratories, and physician offices that already have a collector on staff who can be an observer. If you are a private individual without access to an observer, this course may not be appropriate for you.

How does an observer report completion of the mock collections to MediaLab?

An observer will complete your online checklist and provide his / her contact information. The completed checklist, including the observer's contact information, becomes part of your certification documentation. MediaLab does not validate or audit completed observations. An individual or company that wants to validate your credentials may need to contact your observer.

Am I DOT-certified after completing this course?

Once you have completed the qualification training from MediaLab and documented that you have completed the five required mock collections, then you are qualified to perform DOT urine specimen collections. You will receive a certificate from MediaLab attesting that you have completed qualification training, and you will be able to print out documentation that you have completed the mock collections. The DOT does not issue certificates, licenses, or collector numbers, so you will not receive any materials from the DOT itself.


Group subscriptions for DOT-regulated Urine Collection Training

Group subscriptions to MediaLab are an excellent way to provide qualification training to multiple employees in DOT-regulated urine specimen collection. MediaLab's DOT-regulated urine collection course is available as part of subscription that includes over 40 courses for healthcare facilities and clinical laboratories. Courses cover OSHA safety and compliance, HIPAA privacy, Medicare compliance, and more. Learn more about group subscriptions »

Detailed Course Information

About the Author

Dr. Garland Pendergraph, who is qualified as a Drug Screen Collection Trainer by the Drug and Alcohol Testing Industry Association, is currently Laboratory Director for Doctors Laboratory, Inc, at their Savannah, Brunswick, and Kingsland, GA locations. He has served as Laboratory Director at two hospitals, and as Chairman of the Medical Technology Departments at East Carolina University in Greenville, N.C. and at the Western Carolina University at Cullowhee N.C. He is the author of various books, monographs, and scientific publications. Dr. Pendergraph received a Ph.D in 1969 at the University of North Carolina, with a major in Parasitology and minor in mycology and bacteriology. He is a member of numerous professional organizations including the American Association of Bioanalysts, the American Society of Microbiologists, and is certified by NCA as a Clinical Laboratory Scientist.


  • The student will be able to describe the three types of collections.
  • The student will be familiar with the different types of custody and control forms. And know how to complete them properly.
  • The student will know and understand the correct sequence of a proper drug screen collection.
  • The student will know how to recognize and handle unusual collection situations.

Course Outline

Click on a link in the outline to view a sample page from this course.

  • Introduction
      • Intent of this program
      • Requirements for Retraining
  • Collections
      • Types of collections
      • Standard collection
      • Monitored collection
      • Monitored collection
      • Observed collection
      • Prosthetic devices
      • Observed collection
      • Observed collection
      • In both the monitored and observed collections, the collector MUST be the same gender as the donor.
  • Custody and Control Forms
      • Federal drug testing custody and control form (CCF)
      • Non-federally regulated custody and control form
      • Custody and Control Form
      • Exceptions
      • When a Federally Regulated Custody and Control form is not available, in all situations it is perfec
  • Requirements for the Collection Site
      • Five areas having prerequisites for proper collection
      • Collection site security requirements
      • Other collection site requirements
      • Collection supplies
      • Collection Supplies
      • Rules for collectors
  • Complete and Accurate Documentation
      • Completing the federally regulated custody and control form
      • Completing the federally regulated custody and control form
      • Completing the federally regulated custody and control form
  • Donor Identification
      • Acceptable forms of identification
      • What if a self employed donor cannot produce photo identification?
      • Unacceptable forms of identification
      • Positive identification must be obtained before collection
  • Steps for Typical Urine Collection
      • Steps for Typical Urine Collection
      • Donor Preparation
      • Donor washes hands
      • The collection kit
      • Donor provides the specimen
      • Specimen integrity
      • Specimen integrity
      • Collector documents specimen temperature
      • Donor gives specimen to collector
      • Collector disperses specimen to bottle(s)
      • Collector applies tamper-evidence seals
      • Collector dates seals, donor initials seals
      • Donor completes certification statement
      • Donor completes certification statement
      • Collector packages specimen
      • Distribution of custody and control form
      • If there are several donors waiting to have a drug screen and two or more restrooms are available at
  • Special Collection Situations
      • When problems occur
      • Indwelling Catheter and External Urine Bag
      • Medications
      • Refusal to test
      • Refusal to test
      • Situations not considered as refusal
      • Donor refuses to complete paperwork
      • Shy bladder
      • Shy bladder
      • Shy bladder
      • Observed collection procedure (1)
      • Observed collection procedure (2)
      • Observed collection procedure (3)
      • Observed collection scenarios
      • Observed collection scenarios
      • Observed collection scenarios
      • Observed collection scenarios
      • Broken Security Seal
  • Fatal Flaws
      • Accidental Spills
      • Fatal Flaws and Correctable Flaws
  • Some Final Thoughts
      • Responsibilities and requirements for collectors
      • Differences between Department of Transportation (DOT) and Department of Health and Human Services (HHS) Mandatory Guidelines for Federal Drug Testing...
      • Additional Resources

Department of Transportation (DOT) Federally Regulated Urine Specimen Collection Training Keywords

These are the most common topics and keywords covered in Department of Transportation (DOT) Federally Regulated Urine Specimen Collection Training:

affixed adulterate signage urine legible post-accident responsethe health conceal bladder drug urination initialing adulteration multi-stall handed recollection commode safety donor transportation vial identification medication laboratory container training


Handing over a urine sample cup

Materials needed for specimen collection.

Donor initials the tamper-evident seal

White CCF and samples are sealed in a bag