Diplococci Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Diplococci and links to relevant pages within the course.
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| The carbohydrate utilization reaction seen in the QuadFerm system shown in the picture provides a definitive identification of N. gonorrhoeae: | View Page |
| Review 2 Smith KR, Fisher HC III, Hook, EW III: Prevalence of fluorescent monoclonal antibody-nonreactive Neisseria gonorrhoeae in five North American sexually transmitted disease clinics.J Clin Microbiol 34:1551-1552, 1996We compared a direct fluorescent monoclonal antibody (DFA) test with alternative enzymatic and fermention tests for identifying presumptive gonococcal isolates in a systematic sample from patients attending five sexually transmitted disease clinics in five cities.Fourteen (2.5%) of 556 isolates from three clinics were nonreactive with the DFA confirmatory reagent and reactive by both the Quad-Ferm and Rapid NH tests. The prevalence of DFA-nonreactive Neisseria gonorrhoeae isolates varies geographically and is independent of local methods for the identification of possible gonococci.On the basis of our findings, we recommend that for use in medicolegal and other instances in which a diagnosis of gonorrhea has the potential to have far-reaching effects, it is appropriate to test DFA reagent-nonreactive, oxidase-positive, gram-negative diplococci by alternative methods of gonococcal confirmation.Although the prevalence of such isolates could change, the fluorescent monoclonal antibody confirmation reagents remain useful for many clinical situations. Their ease of use and ready applicability for screening large numbers of isolates make them useful for many laboratories. | View Page |
| The presence of Gram negative intracellular diplococci in a female is diagnostic of gonorrhea. | View Page |
| Identification of bacteria Identification of bacteria in direct smears may be of lifesaving importance. For example, a rapid diagnosis of bacterial meningitis, made after examining a gram stained smear of the patient's cerebrospinal fluid, allows the physician to begin treatment immediately. The appearance of bacteria on gram stained smears is suggestive of a certain species, but identification may not be made on the basis of the stain alone. An exception to this rule is the presence of gram negative intracellular diplococci from a male urogenital specimen, which is presumptive identification of Neisseria gonorrhoeae. In addition, culture results can be correlated with the direct smear report. | View Page |
| Gram negative diplococci When gram negative diplococci with flattened, adjacent sides are seen within white cells, it may indicate the presence of Neisseria. An example of diplococci with flattened adjacent sides, probably representing Neisseria is shown by the arrows. | View Page |
| Reporting Genital Smears Direct smears read specifically for the presence of gonococci should include a direct reference to gram negative intracellular diplococci. | View Page |
| In a male with a purulent urethral exudate, a presumptive diagnosis of gonorrhea is made by finding Gram negative intracellular diplococci in a direct smear of the exudate. | View Page |
| Which of the following appear as budding organisms? | View Page |