Diazonium Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Diazonium and links to relevant pages within the course.
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| Bladder Infections Bladder infections are usually caused by gram-negative bacteria. These bacteria reduce nitrates derived from food to nitrites when urine remains in the bladder three to four hours, sufficient time for this reaction to occur. Nitrite is not present in urine under normal circumstances. When present, nitrites react with p-arsanilic acid to produce a diazonium compound. The diazonium compound in turn couples with 3-hydroxy-1,2,3,4 tetrahydrobenzo-(h)-quinolin to produce a pink color. A first morning, clean, voided midstream specimen is optimal for detecting nitrites in urine. | View Page |
| Granulocytic white blood cells Granulocytic white blood cells in a urine sample suggest the presence of a urinary tract infection. Granulocytes, which include neutrophils, basophils and eosinophils, contain esterases. These esterases catalyze the strip reagent indoxylcarbonic acid ester to release indoxyl. Indoxyl reacts with a diazonium salt to produce a purple color. The intensity of the color produced is proportional to the amount of enzyme present. | View Page |
| Principle of the Ictotest® The reaction for the Ictotest® is based on combining a solid diazonium salt with bilirubin. When bilirubin is present, the reaction results in a blue or purple color. No other color change is considered positive. | View Page |