Coomassie Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Coomassie and links to relevant pages within the course.
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| Automation Automated systems for protein electrophoresis are available for large volumes of samples for electrophoresis. An automated system is capable of separating 10-100 samples simultaneously. There are several different automated systems and the number of process steps automated varies. Automated steps may include reagent addition, sample application, electrophoresis separation, staining, and detection.Below is a stained electrophoresis land for one sample analyzed on the automated instrument, SPIFE 3000, Helena Laboratories. The separated proteins are stained with Acid Blue, a Helena Laboratories' stain developed from Coomassie dye. | View Page |
| Stains and Dyes Substance Stain or Dye Comments Proteins Ponceau S Coomassie Brilliant Blue Silver Specific for Proteins Silver is a biohazard Lipoproteins Sudan Black B Oil Red O - Enzymes Enzyme substrate and a chromagen or fluorescent dye Reaction catalyzed by enzyme and color or fluorescence detected Hemoglobin Not needed Is intensely colored Nucleic Acids (DNA/RNA) Ethidium Bromide (EtBr) SyBr Green Silver EtBr is Carcinogenic SyBr Green is new - Introduced in 1995 Silver is a biohazard | View Page |
| Which statements below are correct descriptions of visualization and detection methods used in electrophoresis? | View Page |