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Candida Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Candida and links to relevant pages within the course.

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Body Fluid Differential Tutorial
Candida albicans - Germ Tube Formation

Candida albicans is known to be a germ tube producer. The elongated, but not quite hyphal, structures that are present in this fungal group are consistent with germ tubes (see arrows).While it is possible to speculate about fungal identity from the cytospin preparation, this is truly the microbiology laboratory's role. This cytospin would be reported as "positive" for yeast and after correlating results with the microbiology laboratory, and according to your laboratory's protocols.

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Yeast

Fungal infections can also be identified in the cytospin preparations from various body fluids. The next series of photos shows the peritoneal fluid from an immunosuppressed patient in renal failure requiring peritoneal dialysis.Note the cluster of yeast cells in the center of the photo. This was eventually identified as Candida albicans.

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Microbiology / Serology Question Bank - Review Mode (no CE)
Which of the following yeasts causes thrush:View Page

Mycology: Yeasts and Dimorphic Pathogens (retired 2/12/2013)
The colonies growing on the surface of this brain-heart infusion with blood agar plate were "converted" from a mold colony suspected of being Histoplasma capsulatum by incubating a subculture at 37°C for 5 days. The yeast forms that must be identified in mounts made from one of these colonies to confirm the identification are:View Page
Match the names of each of the yeast species with its most likely colony morphology as seen in the images on the right.View Page
Match the name of each of the species of yeast listed with its corresponding microscopic appearance as illustrated in the images on the right.View Page
Match the names of each of the yeast species listed with its corresponding appearance when grown in cornmeal agar, as seen in the images.View Page
Match the names of each of the species of yeast listed with its associated phenotypic property that is helpful in establishing a species identification.View Page
Match each of the microscopic structures listed in the drop-down box with the name of its corresponding yeast species on the right.View Page
Match the name of each species of yeast listed below with the location listed in the drop-down box where that species may be concentrated.View Page
Arrange the yeast species listed in the drop-down box in order of increasing virulence, from the least to the most pathogenic.View Page
Match the complications that are most likely to be associated with each of the two yeast diseases that are listed in the drop-down box:View Page
Match each of the fungal species listed below with the appropriate category, indicating whether or not it has the capability of producing pseudohyphae on cornmeal agar.View Page
The colonies shown in this photograph were grown on Guizotia abyssinica (bird seed) agar at 30°C for 72 hours. The most likely identification is:View Page
The growth of the yeast-like colonies shown in the upper image was obtained on blood agar from a skin culture only in the area overlaid by virgin olive oil. The lower image is a photomicrograph of a lactophenol blue mount made from a portion of the colony. The disease associated with this fungus is:View Page
The forms seen in this photomicrograph, produced from a light inoculum of an unknown yeast colony incubated in rabbit plasma at 35°C for 2 hours, leads to the presumptive identification of:View Page
Shown in this photomicrograph is a Gomori methenamine silver stain of a lung biopsy obtained from a patient with X-ray evidence of multi-focal pneumonitis. The yeast most likely to be recovered in culture of this tissue is:View Page
The colony shown in the upper image was recovered from peritoneal fluid of a patient receiving continuous peritoneal dialysis. The lower image is a photomicrograph prepared from a small portion of the colony illustrating the microscopic morphology. Each of the following species of yeast can be eliminated except:View Page
The most common eye complication in patients with candidiasis is:View Page
Illustrated in this photomicrograph of a lactophenol blue preparation of a urine sediment is a cluster of yeast cells that were presumptively identified as Cryptococcus species. Further characteristics that may assist in confirming this identification are:View Page
This photomicrograph is a representative field of a Wright-Giemsa-stained bone marrow aspirate in which a pair of budding yeast cells is seen centrally (arrows). Based on the appearance of these yeast cells, what other test would you expect to be positive?View Page
Oral candidiasis may be directly exasperated by the habitual ingestion of:View Page
The ability of Candida albicans to alternate between two phenotypes, which may be related to the virulence of this species, is called:View Page
A yeast identification system gave a biotype number for an unknown isolate that did not differentiate between Candida tropicalis and Candida parapsilosis. This isolate could be identified as C. parapsilosis in a cornmeal agar preparation if it produced:View Page

Reading and Reporting Gram Stained Direct Smears
This Gram stain was prepared from a sputum specimen and is viewed under oil immersion (1000X). What is the structure that is indicated by the arrow?View Page

The Urine Microscopic: Microscopic Analysis of Urine Sediment
Yeast

Yeast can appear as single cells or in the budding form. As single cells they can be confused with RBCs because they are about the same size. In the budding form, yeast is easily identified as demonstrated on this slide. Yeast can be found in patients with cystitis due to yeast, usually candida, or as a vaginal contaminant from patient's with vaginal candidiasis.

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