Blot Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Blot and links to relevant pages within the course.
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| Manual Urine Reagent Strip Procedure Use a fresh, well-mixed uncentrifuged urine. Hold the reagent strip by the opposite end from the test areas and dip the stick into the specimen so that all test areas are immersed in the specimen. Remove the stick immediately. Prolonged immersion in the sample may wash out the test reagents. Hold strip in a horizontal position and run the edge of the strip against the rim of the urine container or touch the long edge of the strip to absorbent toweling to remove excess urine (do not blot the strip). Maintain the strip in a horizontal position to prevent mixing of reagent chemicals. Observe the reagent pads at the specified time periods. Color changes that occur after the stated maximum read time are not valid. Hold the strip close to the chart and compare the colors to read the results. A good light source facilitates accurate reading. Quality control procedures should be performed with each new lot of reagent strips and as often as required by the laboratory's procedure | View Page |
| A confirmatory test for HIV in patients who are positive by ELISA is the: | View Page |
| Which of the following assays is routinely used for confirmation of HIV infections: | View Page |
| Which of the following techniques is used as a confirmatory test for HIV antibodies: | View Page |
| Which of the following assays is routinely used for confirmation of HIV infections: | View Page |
| Blotting Techniques Blotting techniques were developed to discriminate fragments of nucleic acids. These techniques involve several processes; electrophoresis is one of the processes and is used to separate fragments of DNA and RNA. In Southern blotting (named after Edward Southern) restriction enzymes cut fragments of DNA are separated by AGE or PAGE, transferred to a membrane or blot, and visualized by hybridization with labeled probes.Northern blotting (not named after an inventor but by analogy to Southern blotting) separates RNA. RNA molecules are shorter and have defined lengths; cutting by restriction enzymes is not required. Denaturing conditions are required because of RNA secondary structures. After membrane blotting, the separated types of RNA are visualized with staining or labeled probes.Western blotting (again not named after an inventor but by analogy to Southern blotting)does not separate nucleic acids; it separates proteins in a mixture. The proteins are usually separated with PAGE, transferred to the membrane and visualized with a labeled antibody against the proteins of interest. | View Page |
| Direct Nucleic Acid Testing Some methodologies include: Southern Blot Fluorescent In Situ Hybirdization (FISH) DNA fingerprinting (RFLP) | View Page |
| Direct Nucleic Acid Tests Southern Blot: Employs a restriction endonuclease enzyme to extract DNA from the cells. DNA detection is done using agarose gel electrophoresis.Fluorescent In Situ Hybridization (FISH): Uses RNA Northern Blot or DNA Southern Blot techniques to detect targets of interest in cytology/histology specimens or other nucleic acid variations. DNA fingerprinting: Restriction Fragment Length Polymorphism (RFLP): Cuts long DNA into shorter fragments before detection to isolate changes or polymorphisms. These can either be detected by Southern Blot or by Polymerase Chain Reaction (PCR). | View Page |
| Match the following tests to their appropriate principle: | View Page |
| Small Surface Spills Small blood spills on work surfaces may be cleaned by first laying paper towels on the spill to blot and avoid splattering, and then applying disinfectant. Larger spills will require other methods.Use an approved cleaning method and appropriate personal protective equipment. Be aware of the potential for splatter and contamination. | View Page |
| Bandage the incision Bandage the incision site.Bleeding time in minutes is easily calculated as the total number of blots at 30 second intervals divided by two.
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