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Bloody Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Bloody and links to relevant pages within the course.

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Laboratories Individuals

Cerebrospinal Fluid
Which of the following characteristics are considered abnormal?View Page
Which of the following conditions can cause a sample to be uninterpretable?View Page
Bloody Specimen

When blood is present in a CSF specimen, it is necessary to determine whether the blood is due to a traumatic puncture or to a pathologic condition. There are several clues to help make this distinction: Traumatic tap:More blood is present in tube 1 than in tubes 2, 3, or 4.When sample is centrifuged within one hour, supernatant is clear.Blood clots on standing.Subarachnoid or cerebral hemorrhage:Blood is evenly distributed in all tubes.When sample is centrifuged within one hour, supernatant is pink or yellow.Blood does not clot on standing.

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More on Undiluted Specimens

In an undiluted specimen, count and differentiate red cells and white cells at the same time. You can count red cells on a hand counter and use the differential counter for white cells. If you cannot differentiate white cells from red cells in the undiluted specimen, a plain capillary tube may be filled with crystal violet acetic acid diluent which is subsequently expelled from the tube. A very thin coating of the diluent will remain on the inside of the tube. CSF is drawn halfway up into the tube, which is then rocked back and forth to mix. The hemacytometer is then filled with the fluid containing stained white blood cells and lysed red cells. If cells are numerous and overlapping and it is necessary to focus through several planes in order to see all of the cells, a dilution must be made. When macroscopic appearance is turbid, milky or bloody, a significant dilution is usually necessary.

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In a moderately bloody specimen, 250 cells are counted in 10 squares. The dilution is 1:100. What is the count/microliter?View Page
True or false: if a CSF specimen is bloody due to a traumatic tap, it may be necessary to correct the CSF WBC count.View Page
Red Cells and Lymphocytes

Many red cells and a small, mature lymphocyte are present in this picture. This is typical of fields seen in samples resulting from a bloody tap.

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CLIA Microbiology / Serology Review
A 20 year-old female was admitted into the hospital complaining of 10 to 15 bloody mucous stools per day, fever, gastrointestinal disturbances, abdominal pain, and nausea. The preliminary O & P report went out as "Probable Entamoeba histolytica trophozoites and cysts, confirmation pending." This patient is most likely suffering from:View Page

HIV Safety for Florida
Which of the following does not pose a significant risk for transmitting HIV?View Page

Tuberculosis Awareness for Healthcare Workers
Symptoms

Symptoms of active TB include:coughing for more than 3 weeks,loss of appetite,unexplained weight loss,night sweats,bloody sputum,hoarseness,fever,fatigue,and chest pain.

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