Biosafety Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Biosafety and links to relevant pages within the course.
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| Which of the following are considered engineering controls? | View Page |
| Which of the following secondary barriers are recommended for microbiology laboratories that work with Biosafety level 3 agents (e.g., Mycobacterium tuberculosis)? | View Page |
| Biosafety Levels Laboratory workers who handle infectious materials in the microbiology laboratory should be aware of the work practices, safety equipment, and barriers that will protect them and others in the area from infectious agents. The Centers for Disease Control and Prevention (CDC) and the National Institutes of Health (NIH) created guidelines to assist laboratories in developing safe practices based on the infectious agents that are handled. These guidelines are referred to as Biosafety Levels 1 through 4. Each increasing number represents increased risk, requiring more stringent work practice and increasingly protective safety equipment and barriers. A copy of the Guidelines can be obtained from the CDC or accessed online at:http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm | View Page |
| Biosafety Level 3 Biosafety level 3 practices, safety equipment, and facility design and construction are applicable to microbiology laboratories that work with indigenous or exotic agents with a potential for respiratory transmission, and which may cause serious and potentially lethal infection. Mycobacterium tuberculosis is assigned to this biosafety level. At biosafety level 3, laboratory manipulations should be performed in a Class l or Class ll biosafety cabinet (BSC) or other physical containment device. Secondary barriers include controlled access to the laboratory and ventilation requirements that minimize the release of infectious aerosols from the laboratory. Secondary barriers should include self-closing double-door access and negative airflow into the laboratory. Exhausted air must not be recirculated. | View Page |