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Bicarbonate Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Bicarbonate and links to relevant pages within the course.

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Laboratories Individuals

CLIA Chemistry / Urinalysis Review
Carbon dioxide is predominately found in blood in the form of:View Page
Metabolic acidosis is characterized by:View Page
What condition is indicated by the following blood gas results: Bicarbonate = 32 mEq/L pCO2 = 65 mm Hg pH = 7.28View Page

CLIA General Laboratory Review
Which of the following would not be considered a normal part of a routine electrolyte panel:View Page

Phlebotomy
Plasma electrolytes

Electrolytes are salts dissolved in water, including:Sodium (Na) Potassium (K) Chloride (Cl) Bicarbonate (CO2). Calcium (Ca)

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Semen Analysis
Diluting a specimen for counting on a hemacytometer

Following liquefaction (20-30 minutes), mix the sample manually by swirling the container several times. Thorough mixing is essential for accurate counting. Calibrated automatic pipettes are used to prepare a dilution. Because of the viscosity of semen, the semen should be added to the diluent using a positive pressure pipettor. The dilution often used for routine sperm counts is 1:20 but the actual dilution factor will vary depending on the total sperm count. For high concentration specimens a greater dilution will be necessary. For low concentrations an undiluted or minimally diluted specimen may be required. The appropriate dilution is determined by estimating the concentration needed to do a count of at least 100 cells per side of the loaded hemacytometer. The diluent that may be used for sperm counts on a hemacytometer can be as follows: 5 gm of sodium bicarbonate in 100 ml of distilled water, plus 1ml of formalin (neutral).

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