Bence-jones Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Bence-jones and links to relevant pages within the course.
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| The reagent strip method for protein primarily tests for which type of protein? | View Page |
| Which of the following tests confirms the presence of Bence-Jones proteinuria: | View Page |
| Which of the following methods would be used to confirm the presence of Bence-Jones
protein in the urine: | View Page |
| Bence-Jones proteinuria can be seen in all of the following conditions except: | View Page |
| Which of the following methods may be employed to definitively identify Bence-Jones proteins: | View Page |
| Bence-Jones proteinuria is associated with all of the following conditions except: | View Page |
| The screening test most commonly used to detect Bence-Jones protein in urine is: | View Page |
| Heat and Acid Test for Urinary Protein The heat and acetic acid test is another semiquantitative test used to confirm the presence of protein in urine. It is more sensitive than the SSA test because the pH of the sample is brought close to the isoelectric point of proteins. However, this test is sometimes considered too sensitive because it can detect trace amounts of protein which are considered normal. The heat and acetic acid test gives false positive results with inorganic iodides, benzoin, tolutamide, and proteoses, similar to the SSA test. Bence-Jones protein consists of dimers of either kappa or lambda light chains from immunoglobulins. This abnormal protein is most often associated with multiple myeloma, but can also be found in cases of lymphoma, macroglobulinemia, leukemia, and other malignancies (Balant and Fabre, 1978). Testing for Bence-Jones protein is not part of the routine urinalysis. However, if Bence-Jones protein is suspected, the heat precipitation test or immunoelectrophoresis can be performed on a urine specimen. The heat precipitation test is based on the protein’s unusual solubility properties. Bence-Jones protein precipitates at temperatures between 40ºC and 60ºC (56ºC optimum), but dissolves again at 100ºC. Upon cooling, the precipitate will reappear around 60ºC and will dissolve again below 40ºC | View Page |