Asaccharolytic Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Asaccharolytic and links to relevant pages within the course.
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|Eikenella - Catalase & Oxidase|
Eikenella corrodens (E) belongs to the HACEK group of miscellaneous gram-negative bacilli which includes Haemophilus aphrophilus (H), Actinobacillus actinomycetemcometans (A), Cardiobacterium hominis (C) and Kingella kingae (K). Cytochrome oxidase and catalase are two rapid tests that help separate the several members of this group. Eikenella corrodens shows cytochrome oxidase activity, but not catalase activity. The positive oxidase reaction separates E. corrodens from Haemophilus aphrophilus and Actinobacillus actinomycetemcomitans, which are both negative. A. actinomycetemcomitans is also catalase positive, an additional characteristic separating it from E. corrodens, which is negative. As Kingella kingae is also oxidase positive and catalase negative, other tests are needed for differentiation. K. kingae produces acid from glucose and maltose (E. corrodens is asaccharolytic).
Although not performed that often, the following tests are useful in separating E. corrodens from other closely related members of the HACEK group: KIA showing an alk/alk reaction; Glucose fermentation (-); Reduction of nitrates to nitrites (+); Production of indole (-); Ornithine decarboxylase (+) The positive nitrate reduction reaction eliminates Cardiobacterium hominis, Kingella kingae and other Kingella species. The positive ornithine decarboxylase reaction eliminates Kingella denitrificans (which also denitrifies nitrate to nitrogen gas, a reaction negative for E. corrodens). Eikenella corrodens is asaccharolytic, whereas most other closely related species produce acid from one or more carbohydrates.