Amplify Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Amplify and links to relevant pages within the course.
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| Human Genome Much research has been conducted to identify the alphabet of the human cellular language otherwise known as the human genome. This identification or roadmap of the human genetic material has opened the door to the mainstreaming of molecular diagnostics within the clinical laboratory setting.While the mapping of the human genome project is complete, many times it is not necessary to be able to identify the entire sequence; rather, we can use the specific portion of the code that is unique to the disease or condition in question. These short portions of the genetic molecular sequence or oligonucleotides, can then be used as probes to seek out and detect or amplify the target sequence. | View Page |
| Amplified Nucleic Acid Testing Principle These methodologies use principles that amplify or multiply the target of interest, usually incorporating an enzyme to produce millions or billions of copies in a relatively short time.Some enzymes used in amplification include: DNA ligase DNA polymerase RNA polymerase Reverse transcriptase Alkaline phosphatase Cleavase Note: the steps in amplified testing will vary depending on the target amplified, requirement for thermal cycling and detection techniques. | View Page |
| Thermal Cycling Thermal cycling (thermocycling) uses specific instrumentation to cause variations in temperature during an amplification procedure in order to produce copies of the target of interest (or amplify the target of interest) so that detection can take place.The temperature variations increase to unfold the DNA and then decrease to allow for reconnection or reannealing. | View Page |