Alternately Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Alternately and links to relevant pages within the course.
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| Pulsed Field Electrophoresis Larger fragments of DNA, > 50 kilobases (kb), cannot be separated with AGE or PAGE in routine electrophoresis systems; the gel pore sizes are too small for their migration. Fragment separation can be achieved with alternately applying the power to different pairs of electrodes. The most common method alternates the positive and negative electrodes in cycles during electrophoresis. The DNA fragments must reorient to a new field direction in each cycle. These changing pulses and reorientations separate the large size DNA fragments.Sample runs require longer time periods, some 24 hours or more, special gel boxes, different electrodes and controls for switching the electric fields during electrophoresis. | View Page |
| The hyaline saprobic fungus that has microscopic features similar to the mold form of Histoplasma capsulatum is: | View Page |
| The hyaline saprobic fungus that has microscopic features similar to the mold form of Coccidioides immitis is: | View Page |
| The colonies shown in the upper image were obtained on blood agar from a sputum specimen after 10 days incubation at 30°C. The lower image is a photomicrograph of a lactophenol blue mount made from a portion of the colony. The diagnosis is: | View Page |
| The growth of the colonies shown in the upper image was obtained on blood agar from a sputum specimen after 8 days of incubation at 30°C. The lower image is a photomicrograph of a lactophenol blue mount made from a portion of the colony. The diagnosis is: | View Page |