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Online compliance and continuing education courses for clinical laboratories

Aids Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Aids and links to relevant pages within the course.

Learn more about laboratory continuing education for medical technologists to earn CE credit for AMT, ASCP, NCA, and state license renewal and recertification. Or get information about laboratory safety and compliance courses that deliver cost-effective OSHA safety training and continuing education to your laboratory's employees.

Laboratories Individuals

Cerebrospinal Fluid
CSF Evaluation and Diagnosis

Examination of CSF provides vital information which aids in the diagnosis of a wide variety of disorders: benign disordersmeningitisencephalitisbrain abscesssubarachnoid hemorrhagecerebral infract vs. intracerebral hemorrhagemultiple sclerosisGuillian-Barre's syndromespinal cord tumormalignant disordersleukemia CNS involvementmalignant tumors of the brain or spinal cordmetastasis of malignant tumors

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CLIA General Laboratory Review
Which of the following infectious agents represent the greatest risk to the laboratory worker:View Page
Which of the following sources is most likely to result in an infection from the AIDS virus:View Page
A decrease in which of the following in AIDS patients results in increased susceptibility to infection:View Page

CLIA Microbiology / Serology Review
A decrease in which of the following in an AIDS patient are associated with increased susceptibility to infection:View Page

Current Topics in Clinical Microbiology
Review 1

Rocourt J. Jacquet C. Reilly A.: Epidemiology of human listeriosis and seafoods. International Journal of Food Microbiology. 62:197-209, 2000While rarely diagnosed prior to 1960, more than 10,000 cases of listeriosis were recorded in the medical literature between 1960 and 1982, and thousands more have been reported annually world-wide. This widespread increase in reporting is most likely due to demographic trends and changes in food production, processing and storage, especially the extended cold food chain and the ability of Listeria monocytogenes to grow at low temperaturesL. monocytogenes is a bacterium responsible for opportunistic infections, preferentially affecting individuals whose immune system is perturbed, including pregnant women, newborns, people over 65 years, immunocompromised patients, such as cancer victims, transplant recipients, people on hemodialysis and AIDS patients.Thus, the increasing lifespan and medical progress allowing immunodeficient individuals to survive, partially explains the increasing incidence of listeriosis. Moreover, L. monocytogenes is ubiquitous and can grow at temperatures as low as 0 degrees C. At this temperature growth is very slow.The expansion of the agro-food industry, the widespread use of systems of cold storage and changes in consumers demands have led to a large increase in the pool of Listeria that can cause food-borne infections.

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Fundamentals of Hemostasis
Which of the following is not true in terms of platelet characteristics?View Page
Primary Hemostasis – Platelet Kinetics

Kinetic Processes Specific to Platelets. Adhesion – When platelets adhere to exposed collagen, they take on a characteristic “spiny” shape. Their inherent stickiness, and the aforementioned spiny shape serve to compliment each other during this process. Von Willebrands Factor (vWF) is absorbed by surface receptors on both the platelet and exposed subendothelial tissue, thereby linking the platelets to the tissue. Release – This process occurs prior to aggregation. Platelets dump the contents of their granules (ADP, Serotonin, & Calcium), which aids the upcoming aggregation process by acting as a chemical signal. Aggregation – Platelets physically bind to each other, not just to the exposed subendothelial walls and collagen of the breached vessel. Platelet aggregation requires sufficient chemical signal stimulation. Stabilization (technically part of secondary hemostasis as fibrin is a product of secondary hemostasis)– This process strengthens the platelet plug with the addition of interwoven fibrin strands, ultimately producing a fibrin clot. The durable fibrin clot is the ultimate goal of hemostatic processes.

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HIV Safety for Florida
HIV is known as a retrovirus because:View Page
A person commits a misdemeanor of the first degree by:View Page
Introduction

Acquired Immunodeficiency syndrome (AIDS) is caused by the Human Immunodeficiency virus (HIV). When HIV enters a person's bloodstream, it attacks and kills the T-helper lymphocytes, which are essential to the body in fighting off infections. As these cells are lost, so is the body's ability to fight infection. Possibly months after the initial infecting episode, an infected person develops a mononucleosis-like illness lasting a week or two. A person may then be free of symptoms for years. But as the T-helper cells die, the person becomes vulnerable to many serious infections. The expected mortality is 100%, and there is no vaccine available to develop specific immunity.

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Reporting results

Each laboratory that performs a test indicative of HIV or AIDS shall report to the county health department in less than 2 weeks.To assure the confidentiality of the patient, reporting of HIV infection and AIDS must be conducted using a system developed by CDC or equivalent system.Each person who violates reporting rules may be fined $500 per offense.

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Depending upon the circumstances, a responsible person who fails to maintain confidentiality of HIV test results and test subjects may be charged with which of the following crimes?View Page

HIV: Structure and Replication
HIV is known as a retrovirus because:View Page
Infection of the Host Cell (1)

The double-stranded DNA molecule now migrates to the nucleus of its host cell. Once it reaches the nucleus, a viral enzyme called integrase joins the replicated HIV DNA to the cell's DNA. The viral DNA now becomes one of the cell's chromosomes and is called a provirus. At this point an individual is infected with and is a carrier of HIV but does not have AIDS.

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Laboratory Ergonomics
The Four E's

Education Learn proper techniques for lifting, stretching and limiting repetitive motion Learn how to use ergonomic aids properly and be compliant Exercise Participate in exercise programs Perform gentle stretching exercises prior to your shift Keep physically fitEngineering Redesign your work area by arranging items properly within the work zone. Examine and improve lighting and noise levels whenever possibleEquipment Watch for ergonomic alternatives and make management aware of available options. Wrist support, mouse, keyboards and keyboard drawers, cart, lamps (less expensive equipment) Furniture, chairs, workbenches, desks (more expensive equipment)

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Laws and Rules of the Florida Board of Clinical Laboratory Personnel
Supervisor Qualifications

Licensed as a technologist or meets the requirements to be licensed Completes a Board-approved two-hour course related to the prevention of medical errors that includes root-cause analysis, error reduction and prevention, and patient safety. Meets one of the following: Doctoral degree in chemical science, biological science, or clinical laboratory science + one year of laboratory experience in the category in which licensure is sought, and 25 hours of Board-approved continuing education in supervision and administration Doctoral degree in chemical science, biological science, or clinical laboratory science + one year of laboratory experience in the category in which licensure is sought, and DLM(ASCP) or CLSup(NCA) for all categories or ASCP specialty certification for the category in which licensure is sought Master's degree in chemical science, biological science, clinical laboratory science, or medical technology + three years of laboratory experience including one year experience in the category in which licensure is sought and either 25 hours of Board-approved continuing education in supervision and administration; or DLM(ASCP) or CLSup(NCA) for all categories or ASCP specialty certification for the category in which licensure is sought Bachelor's degree with 24 semester hours of academic science including 8 semester hours of biological sciences and 8 semester hours of chemical sciences + five years of lab experience, of which two must have been as a technologist and at least 1 year experience in the category in which licensure is sought, and either 25 hours of Board-approved continuing education in supervision and administration or DLM(ASCP) or CLSup(NCA) for all categories or ASCP specialty certification for the category in which licensure is sought.

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Technologist Qualifications

Meets one of the following:Bachelor's degree in clinical laboratory, chemical or biological science plus:Completion of a medical technologist training program ORThree years of laboratory experience, at least one of which must be in the applied-for specialtyAssociate's degree plus:Florida technician's license and completion of a technician level medical laboratory training program ORFive years of laboratory experience, at least one of which must be in the applied-for specialtyPasses an examination in one or more specialtiesCompletes one hour of HIV / AIDS continuing educationCompletes two hours of medical errors continuing education

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Technician Qualifications

Meets one of the following:Completes a medical lab technology training program at the technician levelHigh school or equivalency diploma + five years lab experienceAssociate's degree + four years lab experienceBachelor's degree + three years lab experienceBachelor's degree in medical technologyPasses an appropriate examination (certain specialties only)Completes one hour of HIV / AIDS continuing educationCompletes two hours of medical errors continuing education

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Director Qualifications

Effective date March 17, 2008All applicants for a Director license must meet the qualifications for a high complexity laboratory director that are defined in 42 CFR 493.1443 as published on October 1, 2007.A licensed physician may direct a clinical laboratory without a separate laboratory director's license if he / she is certified in clinical pathology by the American Board of Pathology (ABP) or the American Osteopathic Board of Pathology (AOBP); is board-certified in the pertinent laboratory speciality; and/or has four years of pertinent clinical laboratory experience (post-graduate) with two years experience in the speciality that will he/she will direct.A non-physician may obtain a director's license for a specialty area if he / she: Holds an earned doctoral degree in a chemical, biological, or clinical laboratory science Is certified in the pertinent laboratory specialty by an approved national board A director can oversee up to five laboratories.

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Summary of Qualifications

The table below summarizes the qualifications for the four types of clinical laboratory personnel licenses. DirectorPhysician certified in clinical pathology OR Non-physician with: Doctoral degreeCertification in a lab specialtyCompleted course on administrationContinuing education in HIV/AIDS and medical errorsSupervisorOne of the following:Doctoral degree + 1 year experienceMaster's degree + 3 years experienceBachelor's degree + 5 years experienceLicensed as a technologist or meets the requirementsOne of the following:Completed course on administration25 hours of CE in administrationCE in HIV / AIDS and medical errors.TechnologistOne of the following:Bachelor's degree + medical technologist training program OR 3 years experienceAssociate's degree + Florida technician's license and completion of a medical laboratory training program OR 5 years experienceCompleted exam in 1+ specialtiesCE in HIV / AIDS and medical errorsTechnicianMeets one of the following:Completed medical lab technology training programHigh school or equivalency diploma + 5 years experienceAssociate's degree + 4 years experienceBachelor's degree + 3 years experienceBachelor's degree in medical technologyCompleted exam (certain specialties only)CE in HIV / AIDS and medical errors

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Which of the following continuing education courses are required for ALL new clinical laboratory supervisors, technologists, and technicians?View Page

Medical Error Prevention
Systems Failure

System designers sometimes fail to analyze the purposes of medical systems and how best to achieve them. Instead, they revert to traditional ways of doing things. These failures of analysis account for many medical errors. An example is the tendency to encourage reliance on rote memory for performing procedures instead of manuals, job aids, checklists, and computerized protocols.

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Mycology: Yeasts and Dimorphic Pathogens
A hematology technologist observed the intracellular forms seen in the field of view of a Wright-Giemsa-stained peripheral blood smear shown in this photomicrograph. In consultation, the microbiology technologist advised that the form seen most likely represents:View Page
A yeast identification system gave a biotype number for an unknown isolate that did not differentiate between Candida tropicalis and Candida parapsilosis. This isolate could be identified as C. parapsilosis in a cornmeal agar preparation if it produced:View Page

OSHA Bloodborne Pathogens (updated October 2008)
How common is HIV?

There are approximately 1.1 million individuals in the United States who are estimated to have HIV/AIDS.Worldwide, it is estimated that there are over 33 million persons with HIV/AIDS, with most of these individuals living in sub-Saharan Africa.As of December 2001, there have been 57 documented cases of occupationally acquired HIV infection among healthcare personnel in the United States since reporting began in 1985 (CDC data).Considering the large numbers of HIV positive patients cared for, this is a very small number.

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What happens after HIV infection?

Days to weeks after exposure, the patient may begin to complain of fever, headache and fatigue. This may also be accompanied by a rash.For the first several months after the infection, the exposed individual maybe HIV antibody negative - this is called a "window" period.The disease may remain silent in the patient for months to years even with no treatment.At some point in time, when the immune system is weakened enough, the patient will develop opportunistic infections and be classified as having AIDS (acquired immunodeficiency syndrome).

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Parasitology Review
The ameba that lives in the gumline of the mouth is known as:View Page
Immunocompromised patients, such as those with AIDS are at an increased risk of contracting which of the following conditions?View Page
Match each parasite pictured with its respective classification:View Page
Match each group of helminths listed with its respective key characteristic that aids in group identification:View Page
Match each parasite listed here with its respective classification:View Page
Adult intestinal roundworms are equipped with this structure that serves as a protective outer layer:View Page
Match each parasite listed here with the key characteristic that aids in its identification:View Page
Match each parasite with the key characteristic that aids in its identification: (Each answer may only be used once.)View Page
A 50 year old male domestic airline pilot was rushed to the hospital after complaining of tremendous fluid loss due to severe diarrhea. History revealed that the patient was diagnosed with AIDS 6 months ago. The doctor ordered a battery of tests including a stool for parasite examination. Since the sample was properly labeled indicating that the patient was immunocompromised, the lab performed both the standard processing procedures and a modified acid-fast (mod AFB) stain. The mod AFB stain revealed this suspicious form which measured a mere 4 µm. This patient is most likely infected with:View Page
I reside in red blood cells where I assume these characteristic appearances:View Page

Routine Venipuncture
Syringe

The syringe and needle combination should be the last equipment option that is considered; it is not as safe a choice as the self-contained blood collection systems because it involves more manipulation. However, the phlebotomist may choose to use a syringe to prevent vein collapse if the phlebotomist thinks that the vein is too fragile to withstand the pressure exerted by the vacuum as it pulls blood into the collection tube. A transfer device aids in the safe transfer of blood from the syringe into blood collection tubes. During blood transfer, do not manually push plunger as this may cause hemolysis of the specimen.

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The Disappearing Antibody: A Case Study
Signs and symptoms - Job Aids

Some blood safety standards require that a list of common signs and symptoms of suspected adverse reactions be included in both nursing and transfusion service manuals. Several organizations have developed job aids to help clinical staff recognize the signs and symptoms of various suspected transfusion reactions and to suggest appropriate actions (e.g., see REACT in Online Resources).

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