Agglutinin Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Agglutinin and links to relevant pages within the course.
Learn more about laboratory continuing education for medical technologists to earn CE credit for AMT, ASCP, NCA, and state license renewal and recertification. Or get information about laboratory safety and compliance courses that deliver cost-effective OSHA safety training and continuing education to your laboratory's employees.
| Initial Steps for Identifying an Antibody Reaction PhaseLook at the phase in which reactions are occuring and see if the results match the reaction patterns on your panel. Reactions occurring only at immediate spin phase could indicate a possible IgM antibody, cold agglutinin, or rouleaux. Reactions occurring in the AHG phase could indicate a possible IgG antibody. Reactions occurring in both reaction phases could indicate a combination of both IgM and IgG antibodies or a strong IgM antibody that carries through to AHG phase. If a tube method is used, reactions are usually read at immediate spin and AHG phase. If a gel method is used, reaction readings are done only at AHG phase. PatternComparing the pattern of reactivity of the sample reactions with that of the panel cells will help to determine which antibody or antibodies are present. If the reactions match, or closely match, a specific panel cell, that could be the antibody.The strength of the reaction does not correlate with the clinical significance of the antibody present. It does correlate to the amount of antibody that is available to react. If reactions are strong at immediate spin (IS) phase and get weaker at antihuman globulin (AHG) phase, it is possible that a strong IgM antibody is present that is carrying through to the AHG phase (e.g., 4+ at IS and 1+ at AHG).The presence of multiple antibodies should be considered if reactions vary in strength or there are two separate reaction patterns in the IS and AHG phases. | View Page |
| Cold antibodies Most are IgM and not clinically significant May interfere with detection of clinically significant antibodies if they react at AHG phase. Screen cells and panel cells will have positive reactions in IS phase and strength will diminish or antibody will not be detected at AHG phase. Auto control will be positive if the cold antibody is an autoantibody. Binding of antibody to antigen occurs at room or colder temperatures and may start to disassociate from the red cell membrane at warmer temperatures. Reactions will appear weaker or be negative at warmer temperatures. (Example: 4+ at IS phase and W (weak)+ at AHG phase.) PrewarmingIf a non specific cold antibody or cold agglutinin is suspected, warm the sample and testing reagents, including saline, to 37° C. Only do reaction readings at AHG; bypassing the optimum reaction temperature prevents activation and binding of the cold antibody . | View Page |
| Which of the following conditions is most frequently associated with anti-I: | View Page |
| The most severe acute hemolytic transfusions reactions are the result of which of the following: | View Page |
| The RBCs found in this illustration are the result of: | View Page |
| Which of the following serological tests would be used for the diagnosis of Q-fever: | View Page |
| The arrangement of erythrocytes on this peripheral blood smear may be seen in each of the following conditions except: | View Page |
| The arrangement of the erythrocytes in this peripheral smear should be reported out as rouleaux formation. | View Page |
| The blood study from which this smear was obtained revealed an MCV of 115 femtoliters (fl).Normal MCV values in adults= 80 - 90 fl.Normal MCV values in full-term infants= 98 -108 fl.Which of the following conditions may be indicated by the results seen on this peripheral blood smear? | View Page |