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Acetest Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Acetest and links to relevant pages within the course.

Learn more about laboratory continuing education for medical technologists to earn CE credit for AMT, ASCP, NCA, and state license renewal and recertification. Or get information about laboratory safety and compliance courses that deliver cost-effective OSHA safety training and continuing education to your laboratory's employees.

Laboratories Individuals

Chemical Screening of Urine by Reagent Strip
A test that can be used as a confirmatory test for urobilinogen is:View Page

Confirmatory and Secondary Urinalysis Screening Tests
Detection of Ketone Bodies

“Ketone bodies” is a generic term which refers to acetoacetic acid (diacetic acid), acetone, and beta-hydroxybutyric acid. Screening procedures used to detect ketonuria do not react with all ketone bodies. Since all three of the ketone bodies will be present in the urine and are equally significant, it is sufficient to detect an increase in any one or two of the ketone bodies. Most procedures, including Acetest®, measures acetoacetic acid and acetone but not beta-hydroxybutyric acid.

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The Acetest®

Urine to be screened for ketone bodies should be tested immediately or refrigerated in a closed container since acetone is lost to the air if the sample is left standing at room temperature for any length of time. The Acetest® can be used for the semiquantitation of ketones in urine, serum, or whole blood, however the reaction times differ depending on the type of specimen tested. The same substances which interfere with the dipstick tests for ketones will also interfere with Acetest® because the same reaction is involved.

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