Absorbance Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Absorbance and links to relevant pages within the course.

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CLIA Chemistry / Urinalysis Review
Another term for absorbance is:	View Page
A spectrophotometric scan of amniotic fluid may be valuable in the determination of which of the following conditions:	View Page
Match principle with instrument:	View Page

CLIA General Laboratory Review
Which type of spectrophotometric blank should be used to account for absorbance differences caused by the specimen being tested:	View Page
The direct relationship between the concentration of a substance and its absorbance is referred to as:	View Page
Spectrophotometric absorbance is related to % transmittance in what way:	View Page

Linear Regression Analysis
A Regression Analysis Example For example, to find a relationship between glucose concentration and absorbance, we could first plot all the points on a scatterplot. Glucose (mg/dL) Absorbance 50 .10 100 .20 150 .30 200 .40 250 .50 300 .60	View Page
Calculating the Y-Intercept To find the y-intercept, calculate and , the average of the x- and y-values respectively. Then substitute these two values for x and y in the = b + a equation. Finally, solve for the unknown quantity a. Therefore, the complete relationship between glucose concentration and absorbance for the data is y = 0.002x, where y is the absorbance and x is the glucose concentration.	View Page
Prediction Using the Resulting Equation Once the parameters have been calculated, the resulting equation can be used to make predictions about a value of y given a value of x, provided that the x value is in the same range of x-values that were used to derive the equation. For example, if x = 350 mg/dL, what is the expected value of y? To find the answer, substitute the known value of x into the equation. When the concentration is 350 mg/dL, we expect the absorbance to be about 0.7.	View Page
Given the following creatinine standards: mg/dL Absorbance 3 0.14 6 0.26 9 0.38 What is the correct form of the regression line?	View Page
Given the data and linear regression line you calculated on the previous question, what is the expected absorbance of a 10 mg/dL sample?	View Page